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GMU:DIY Biolab “Driver’s License”/Eva Walch (view source)
Revision as of 22:00, 14 February 2018
, 14 February 2018→BACTERIA INTERACTION WITH PAINTING
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===''' | ==='''Media experiments:'''=== | ||
In these tries, I was starting to figure out how I can let grow diffenent kind of bacteria. | |||
One way is to change the ph-level of the medium | |||
(source: Wikipedia [https://de.wikipedia.org/wiki/Selektivnährboden]): | |||
"Unter Selektivnährboden versteht man einen Nährboden, der das Wachstum einer bestimmten Gruppe von Mikroorganismen, beispielsweise bestimmter Bakterien, begünstigt während er die Entwicklung anderer Mikroorganismen unterdrückt. Dies geschieht aufgrund der Zusammensetzung des Nährbodens, da Mikroorganismen unterschiedliche Bedürfnisse hinsichtlich des Kulturmediums in puncto Nährstoffzusammensetzung, '''pH-Wert''' etc. besitzen und da einige Stoffe andere Mikroorganismen in ihrem Wachstum hemmen." | "Unter Selektivnährboden versteht man einen Nährboden, der das Wachstum einer bestimmten Gruppe von Mikroorganismen, beispielsweise bestimmter Bakterien, begünstigt während er die Entwicklung anderer Mikroorganismen unterdrückt. Dies geschieht aufgrund der Zusammensetzung des Nährbodens, da Mikroorganismen unterschiedliche Bedürfnisse hinsichtlich des Kulturmediums in puncto Nährstoffzusammensetzung, '''pH-Wert''' etc. besitzen und da einige Stoffe andere Mikroorganismen in ihrem Wachstum hemmen." | ||
Here I tried to cook media with different kind of acidity | |||
#in the first petri dish there is the medium for the Growth of Gluconacetobacter but without citric acid | #in the first petri dish there is the medium for the Growth of Gluconacetobacter but without citric acid | ||
#in the second petri dish there is the standart medium for the growth of Gluconacetobacter, which includes some citric acid | #in the second petri dish there is the standart medium for the growth of Gluconacetobacter, which includes some citric acid | ||
#the third petri dish contains the medium for the growth of Gluconacetobacter, but with the double the amound of citric acid | #the third petri dish contains the medium for the growth of Gluconacetobacter, but with the double the amound of citric acid | ||
<gallery> | |||
File:VerschiedeneMedien.jpg | |||
File:Nährböden.jpg | |||
File:Nährböden2.jpg | |||
</gallery> | |||
So I found out, that to less acidity there is nothing really happening and with more acidity than usual there are no more bacteria growing, but fungi. | |||
What I also realized, is that you can play with the different kind of sugar you are using to cook the medium. | |||
It makes a big different, if you use glucose or fructose, but also you can use for example potato starch. | |||
<gallery> | |||
File:FructoseMedium.jpg | |||
</gallery> | |||
furthermore I was trying to extract two different bacteria into two different petri dishes to have to clean bacteria to be able to paint with them | |||
<gallery> | |||
File:extractedBacteria.jpg | |||
File:extractedBacteria2.jpg | |||
File:extractedBacteria3.jpg | |||
</gallery> | |||
==='''Coloring bacteria and media:'''=== | |||
In this experiment I try to color bacteria after they grown, by putting red pigment to the bacteria | |||
<gallery> | |||
File:RotePigmente.jpg | |||
</gallery> | |||
after one week: | after one week: | ||
<gallery> | |||
File:Rot1.jpg | |||
File:Rot2.jpg | |||
</gallery> | |||
Here I was putting ink into the medium, so that it became blue. But after heating it up in the microwave the blue color disappeared. | |||
But still the bacteria were growing different than on the normal medium, somehow more intense, maybe because there is more bakteria inside with the added ink | |||
<gallery> | |||
File:InkMedium.jpg | |||
</gallery> | |||
in | in the second try, I put ink into the medium, but after the heating in the microwave | ||
<gallery> | |||
File:ink.jpg | |||
File:ink2.jpg | |||
File:ink3.jpg | |||
</gallery> | |||
It looks like that the bacteria is growing much faster on the part of the medium, where there is no ink. But also where the ink is there are growing bacteria. | |||
It also seems like they are soaking in the color. | |||
Further steps: | ===Further steps:=== | ||
Now it would be interesting to let grow different bacteria in the same petri dish. Therefore I would use different kind of media, that means using different kind of sugar, and also playing with the acidity. | |||
Furthermore I could choose between the extracted bacteria, depending on what kind of bacteria I want to have on which part of the petri dish. | |||