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GMU:Https://www.uni-weimar.de/kunst-und-gestaltung/wiki/index.php?title=GMU:DIY Bio: doing things with biology/Sirin Unmanee/30.10.2018 : Isolating Bacteria from Kombucha, Growing Physarum polycephalum (2nd attempt) and others&action=edit&redlink=1: Difference between revisions
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'''Isolating Bacteria from Kombucha''' | |||
Here is list of ingredients to prepare 500 ml of liquid ''Gluconcetobacter'' medium | Here is list of ingredients to prepare 500 ml of liquid ''Gluconcetobacter'' medium | ||
Glucose - 10 g | Glucose - 10 g | ||
Peptone - 2.5 g | <br />Peptone - 2.5 g | ||
Yeast extract - 2.5 g | <br />Yeast extract - 2.5 g | ||
Na2HPO4 - 1.35g | <br />Na2HPO4 - 1.35g | ||
Citric acid - 0.75 g | <br />Citric acid - 0.75 g | ||
Distilled or tap water - 500 ml | <br />Distilled or tap water - 500 ml | ||
Agar - 7.5 g | <br />Agar - 7.5 g | ||
However, in this case, I needed just 100 ml, so everything is divided by 5. | |||
I dissolved 2 ml of Kombucha and 8 ml of distilled water, then smeared one drop of that solution onto the first slot. The others' solutions are dissolved in 1/2, 1/4, 1/8, 1/16 and 1/32 ratio respectively. | |||
[[File:S__3178602.jpg|400px]] | |||
'''Growing Physarum polycephalum (2st attempt)''' | |||
I failed growing slime mold, so I tried once again with three dishes (just in case!), but this time I keep all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing the slime mold. | |||
'''Growing Physarum polycephalum (2st attempt)''' | |||