GMU:DIY Biolab “Driver’s License”/Mana Mazidy

Glowing Banknote tell the true story.

A banknote is a document that serves as a means of payment in a particular country. The banknote is a small poster that tells the story of a country. In this project we try to show that the true story is hidden behind the beautiful photo. Behind the laughing faces of politicians, men scream for help, crying the mothers and trembling children. Here you will not see famous politicians, people laughing and beautiful nature, but you can see the sad story that is happening right now!

The banknotes are public media. it means everyone knows them! You do not need to go to the museum to see them. that's why we think of it as a very powerful medium and want to use it to tell the true story of the countries!

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But how?

The publishers of banknotes try to make their banknotes difficult to copy. They use different techniques to control the correct banknotes One of the techniques is: Fluorescent colors It is used printing ink containing fluorescent pigments. In short-wave UV light, depending on the choice of pigment, colors are emitted in the visible light spectrum. In this project instead of pigments we use bacteria to draw our pictures. So we have a connection between human, media and bacteria!

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Which countries Which banknotes

we want to tell the story of the countries where currently in 2017-2018 goes wrong. War. Diseases . Poverty ... In this time there are many countries where there are war or countries in which the children starve to death. Some countries are economically blocked and do not get any medicines, so many die after illnesses.

syrien irak myanmar yemen

Which medium ??

bioluminescence

Bioluminescence is the ability of a living organism to produce and emit light, so to send it out. This is possible through certain chemical reactions that take place within the organism. A luminescent substance (luciferin) is oxidized to oxyluciferin with the help of an enzyme (luciferase) (consumption of oxygen and energy). The decomposition of oxiluziferin produces energy that is converted into light of a specific wavelength (λmax 530 nm) almost without loss. Due to the wavelength, we perceive the glow as green.

distribution

Animals

mushrooms

protozoa

bacteria

In this project I use mushrooms and bacteria to illuminate the drawings

bioluminescent chemicals

So we have a connection between humans, media and living organism. You can see the normal photos on banknote, but when the light is off, the bacteria will glow and you'll see glowing photos telling the right story.

bacteria

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Ability

they only glow when they are alive . so you can know are they dead or alive ! in this project we can use this Ability to draw . The positive area should be filled with live bacteria and negative area with dead bacteria.

Make Bacteria glowing :

bacteria need food to grow It should to be full of vitamins, amino acids, carbon, minerals and of course salt, without salt they won't live !

Agar is made from the cell walls of some algae species , mainly from East Asia. In microbiology, microbial growth media are almost invariably solidified with agar instead of the gelatin used until the end of the 19th century. Agar is more resistant to gelatin than the high temperature used in sterilization, and gelatin gels become liquid at higher incubation temperatures. Agar gel becomes liquid at 95 ° C and is more stable at higher temperatures than gelatin gels; it solidifies at 45 ° C. Thus, the addition of thermolabile substances before solidification is possible. Because some microorganisms can digest gelatin, this is another reason.

6.Desember

I tryed to Make Some Bacteria Food :

TSA agar gel*

what I need : NaCI 2g . Yeast Extract 1g . Peptone 2g . Agar 3g . Fill with H2O up till 200 ml

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Photobacterium

What ist Photobacterium

Tehy are a genus of Gram-negative bacteria and belong to the family of Vibrionaceae. Some members of this genus have the ability to produce light and are therefore bioluminescent

7.Desember

it is not glowing :(

14.Desember

they are growing but not glowing

20.Desember

I use this bacteria to draw

I want to see how many details are visible when they are growing

The illuminated mushroom

This mushroom has developed an amazing feature, it shines in the dark. In our native forests sometimes infected by the Hallimasch root pieces. This phenomenon is referred to as bioluminescence and is also known by the fireflies. For most observers of yesteryear, the glowing wood was mysterious and eerie, and associated with wizards, fairies, and elves. In this mushroom species shine both the mycelium and the fruiting bodies. Interestingly, however, only the American style of Panellus stipticus shines. He has a creamy white color and 1-2 cm large, shell-like fruiting bodies that spread throughout the trunk. Every year new fruiting bodies appear.

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Nothing happens without reason in nature. So the extraordinary appearance of the mushrooms has a biological function. The emitted green light is visible to insects and attracts them. Similar to the "bees and flowers principle", the insects land on the mushrooms and transport on their onward flight spores that have stuck to them. Thus, the mushrooms receive by their striking luminous advantages in the propagation and distribution.

Panellus stipticus The light mushroom (Panellus stipticus), often called fairy-fire or ghost-mushroom, belongs to a small group of mushrooms, which glow in the dark. Through natural bioluminescence, similar to fireflies, the tormenting twining (Panellus stipticus) enlightens its surroundings in a unique light. The mycelium is already recognizable, but the fruiting bodies are even more intense.

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The mushroom is also ideal for terrariums with reptiles, amphibians and invertebrates, if they are regularly moistened ......................................................................................

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Same projects:

[7]https://bscd.uchicago.edu/news/student-project-glowing-bacteria-art-gallery

Student project of chicago university fluoresced bacteria - meta-bacterial

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[8]http://flavorwire.com/398549/glow-in-the-dark-art-created-in-the-lab-with-bioluminescent-bacteria/view-all

With Bioluminescent Bacteria - gallery at Montana State University

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[9]http://www.miyaando.com/installations-1/2016/12/8/amwdv9kbdzbcliqsfs211iazo1pdiy

from artist Miya Ando and photographer L. Young. With a different kind of bioluminescence

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[10]http://2012.igem.org/Team:Caltech/Human_Practice

California Institute of the Arts to create animations from fluorescent bacteria

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[11]https://www.huffingtonpost.com/entry/agar-art-contest_us_56253e13e4b02f6a900d3d57

composed of multiple petri dishes of bacteria modified with fluorescent protein under UV light

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Modification of green fluorescent

I noticed that most artists used either bioluminecenses or fluresences bactera.

But what's new: modified with fluorescent protein! I tried to know, what is it? and how can I do that?

is a protein composed of 238 amino acid residues and shows a bright green fluorescence when exposed to blue to ultraviolet light. The artists have used it for art projects and scientists have used it to highlighting specific structures.

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Different proteins produce different fluorescent colors when exposed to ultraviolet light. (Picture taken by Erik A. Rodriguez.) https://en.wikipedia.org/wiki/Green_fluorescent_protein#/media/File:Fluorescence_from_Fluorescent_Proteins.jpg

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