1st class: 16. Oktober 2018 - Introduction
Summary
General introduction to working in the bio lab. Preparing the first testing Agar-Plates for bacterial growth in a petri dish:
- petri dishes where filled with 15 ml of an LBP (Baird-Parker liquid) Medium with Agar Agar
- cooling down
- inoculateing with different surface smear
- In my case: human nose (inside), cat nose (outside) and fur, fridge glass surface and 1 cent coin
- left with room temperature for the following days
First culture medium
As described obove the filled petri dishes are now beeing observed for the following days.
Gallery
Day 1
Day 3
Day 6
Day 7
Breeding day 7 - closer look
Somehow I got the impression that the petri dishes weren't sterile as after 7 days almost the full dish is covered with a white film. You can only guess by slightly different colors where I initially inoculated the dish. Something seems to grow over the originally inoculated sections. See below some close ups. In the section of the example I took from my nose, a small yellow dot appears. I think this is what was intended to be growing. The rest just looks almost the same.
Gallery
Cat fur and outside nose
1 Euro cent
Day 7 - Full dish
Human nose inside
glass surface fridge
DIY Microscope Test
What was done?
- extracted converging lens from a laser pointer
- attached to iPhone SE back camera
Results
Chestetree seed
Sunflower seed
Sunflower and chestetree seed in comparison
Chestetree seed magnified
Sunflower seed magnified
- adding some filters to increase contrast und depth
Artsy chestetree seed
Artsy sunflower seed
Literature
László Moholy-Nagy
From material to architecture (Full text english)
(Source: https://monoskop.org/images/a/af/Moholy-Nagy_Laszlo_The_New_Vision_and_Abstract_of_an_Artist.pdf, page 14 - 19)
Von Material zu Architektur (Volltext deutsch)
(Quelle: https://monoskop.org/images/b/b5/Moholy-Nagy_Laszlo_Von_Material_zu_Architektur.pdf, Seite 10 - 18)
Dimitris Papadopoulis
Generation M - Matters, Makers, Microbiomes (source: https://dialnet.unirioja.es/descarga/articulo/4903686.pdf)
2nd class: 23. October 2018 - Ethics - Microscopy - Physarum Polycefalum
Summary
We first discussed two papers we read at home. One from Moholy-Nagy and the other one from Dimitris Papadopoulos. Moholy Nagy refers to the primitive human being and how he considered the human beeing back in the age when he lived. Paper number two
Today we've been talking about ethical questions that come up while working living tissues. To sum it up: At all times you always need to be aware of working with living organisms. Be respectful and know that you are always responsible for what you do and that, in the end, you have to deal with the consequences.