CULTIVATION OF Haematococcus Pluvialis.
- Known by its red color, it's an alga that in stress conditions produces a high content of a strong antioxidant known as astaxanthin.
* SCIENTIFIC CLASIFICATION Division: Chlorophyta Class: Chlorophyceae Order: Chlamydomonadales Family: Haematococcaceae Genus: Haematococcus Species: H. pluvialis Binomial name Haematococcus pluvialis
https://en.wikipedia.org/wiki/Haematococcus_pluvialis
Sequence of growth states of the Haematococcus: https://comunicarciencia.bsm.upf.edu/?tag=haematococcus-pluvialis
- MEDIUM
- 100ml of water
- 3% of vinasse
- 0.7% of NaCl.
The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture.
Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium Malz extract. (brennwert: 1.216kJ, fett 0.2g, kohlenhydrate 65g, Zucker 45g, Eiweiss 5g, Salz0.02g.)
- Day 1: Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH 7. Preparation and from left to right; original medium, 10% of original medium diluted in 100ml of water, 1% of original sample diluted in 100ml of water.
The three samples contain:
1 Sample 3,00g malz extract, 100ml water & 0,7g NACl. 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl. 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.
- Day 2: An observation under the microscope is made in order to see the specimen.
((Ask for the photos... made day 3))
- Day 4: After 4 days of growth the specimens don't show any change of state. At the moment it would be expected that they start to acquire an orange color (if the conditions were ideal for the specimen). A hypothesis is that the medium is not the one that they need in order to survive, or that the conditions of light and warm are not ideal for them.
- Day 14: After 10 days the Haematococcus is settling in the bottom of the tube, it didn't acquire any other color, what it means that it conditions were not ideal for them to develop.
A new medium for the specimen must be done, and further investigation has also to be done, in order to find the ideal conditions to cultivate it and for it to grow. (For the moment the specimen is in a hibernation state.