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After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test. | After the preparation was finished, we loaded these samples in one neat row on a gel bed connected to electrodes. After half an hour the florescent light showed that she samples assembled themselves in columns at various lengths. We used the raspberry as our control and the raspberry sample columns all assembled themselves to the same hight as the control. We could determine which samples are of plant and which of animal origin. Very few samples were successfully put through this PCR test. | ||
[[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]] | [[File:20230428 134817.jpg|thumb|1082x1082px|Preparing samples 2[[File:Results.jpg|thumb|1548x1548px|Results]]]] | ||
[[File:20230521 115319.jpg|thumb|341x341px|Setting up technology]] | [[File:20230521 115319.jpg|thumb|341x341px|Setting up technology[[File:Nanopore active cells.jpg|thumb|345x345px|Nanopore active cells]]]] | ||
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20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing. | 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing. | ||
Once in the laboratory we extracted small amounts of the fluid from multiple cyanobacteria and algae cultures. We then prepared the sample by adding various buffers according to the prescribed protocol. | |||
The sample contained many organisms and once the sequencing was done we could compare the results to the information in online libraries and identify the organisms present in our sample. | |||