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'''WORKSHOP III.''' | '''WORKSHOP III.''' | ||
20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing. | 20.05.2023, 21.05.2023 - [[GMU:BioArt Forum/Nanopore sequencing|/Nanopore sequencing]] with safety engineer Lisa Thalheim[[File:MinION sequencer with flow cell.jpg|thumb|340x340px|MinION sequencer with flow cell that is being loaded with the sample[[File:Comparing the results with online libraries.jpg|thumb|345x345px|Comparing the results with online libraries]]]]Lisa gave us a beautiful overview of an Artist's Guide to DNA Sequencing and how the technology was previously used by artists. After the introduction we were acquainted with the Oxford Nanopore Technologies (https://nanoporetech.com/) and the equipment we later used for DNA sequencing. | ||
Once in the laboratory we extracted small amounts of the fluid from multiple cyanobacteria and algae cultures. We then prepared the sample by adding various buffers according to the prescribed protocol. | Once in the laboratory we extracted small amounts of the fluid from multiple cyanobacteria and algae cultures. We then prepared the sample by adding various buffers according to the prescribed protocol. | ||
The sample contained many organisms and once the sequencing was done we could compare the results to the information in online libraries and identify the organisms present in our sample. | The sample contained many organisms and once the sequencing was done we could compare the results to the information in online libraries and identify the organisms present in our sample. | ||