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GMU:Life in an aquatic ecosystem/Dahye Seo (view source)
Revision as of 11:43, 17 November 2024
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TBE Buffer for cover gel : 50 ml TBE Buffer, 1.25 µl DNA Stein green | TBE Buffer for cover gel : 50 ml TBE Buffer, 1.25 µl DNA Stein green | ||
Gel Electrophoresis : 5V - 40 min -> 5V - 40 min -> 6V - 60 min | Gel Electrophoresis : 5V - 40 min -> 5V - 40 min -> 6V - 60 min (current 400 in every process) | ||
The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles. | The instructions say to electrophoresis at 5v for 40 min. Due to the short distance of DNA migration, I did 2 additional cycles. | ||
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<nowiki>*</nowiki>DNA moves slow. Next time, reduce the percentage of agarose gel ? | <nowiki>*</nowiki>DNA moves slow. Next time, reduce the percentage of agarose gel ? | ||
How to set up the current for the gel electrophoresis? Does it impact the value of current for the movement of DNA? | |||