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GMU:Life in an aquatic ecosystem/Dahye Seo (view source)
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How to set up the current for the gel electrophoresis? Does it impact the value of current for the movement of DNA? | How to set up the current for the gel electrophoresis? Does it impact the value of current for the movement of DNA? | ||
2024.11.18. Mon | |||
<u>Introduction</u> | |||
The purpose of this experiment is to find out how DNA migrates when exposed to UV light throughout the whole gel electrophoresis process. | |||
<u>Procedure</u> | |||
A 100 bp DNA ladder was electrophoresed at 50 volts, 40 minutes. | |||
<small>* Note</small> | |||
<small>1. Reused the gel from 2 days ago.</small> | |||
<small>2. Did not put stain green in the TBE buffer covering the gel.</small> | |||
<u>Result</u> | |||
When the UV was shined throughout the experiment, the DNA movement is very blurry compared to when it was not. | |||
However, I am not sure if this is due to the UV light or the reuse of the gel or the lack of stain green. | |||
<gallery> File:DNALadder UV lighting.jpg </gallery> | |||