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GMU:BioArt WS16/Transgenic organisms (view source)
Revision as of 08:16, 4 January 2017
, 4 January 2017→GFP E.coli Workshop (method 1)
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* (Reptilian) incubator | * (Reptilian) incubator | ||
* Spectrometer to calculate cell growth mass | * Spectrometer to calculate cell growth mass | ||
TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the | TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water. | ||
Day 2 | Day 2 | ||