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GMU:BioArt WS16/Transgenic organisms (view source)
Revision as of 12:06, 4 January 2017
, 4 January 2017→GFP E.coli Workshop (method 1)
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Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night | Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night | ||
Day 3 | |||
https://www.google.de/search?q=gfp+e+coli&tbm=isch | |||
== References == | == References == | ||
* wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis | * wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis | ||
* wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting | * wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting | ||