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==Algae== | ==Algae== | ||
===Pinnularia=== | ===Pinnularia=== | ||
[[File:Pinnularia_biologydiscussion_com.jpg|400px|thumb|Pinnularia. Source: biologydiscussion.com]] | |||
====Classification==== | |||
*Domain: Eukaryota | *Domain: Eukaryota | ||
*Phylum: Heterokontophyta | *Phylum: Heterokontophyta | ||
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*Genus: Pinnularia | *Genus: Pinnularia | ||
====Description==== | |||
Pinnularia is a fresh water alga, a type of diatom (phytoplankton/Bacillariophyceae) which is lives in oceans, in fresh water, in soils, and on damp surfaces. “Diatoms are unicellular, although they can form colonies in the shape of filaments or ribbons (e.g. Fragilaria), fans (e.g. Meridion), zigzags (e.g. Tabellaria), or stars (e.g. Asterionella).” “Diatoms generally range in size from 2 to 200μm” (wikipedia) | Pinnularia is a fresh water alga, a type of diatom (phytoplankton/Bacillariophyceae) which is lives in oceans, in fresh water, in soils, and on damp surfaces. “Diatoms are unicellular, although they can form colonies in the shape of filaments or ribbons (e.g. Fragilaria), fans (e.g. Meridion), zigzags (e.g. Tabellaria), or stars (e.g. Asterionella).” “Diatoms generally range in size from 2 to 200μm” (wikipedia) | ||
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“Pinnularia is a predominantly fresh-water alga, usually found in ponds and moist soil. They can also be found in springs, estuaries, sediments, and oceans. Members of this genus are most commonly found in 40 cm of water, at 5 °C.” (wikipedia) | “Pinnularia is a predominantly fresh-water alga, usually found in ponds and moist soil. They can also be found in springs, estuaries, sediments, and oceans. Members of this genus are most commonly found in 40 cm of water, at 5 °C.” (wikipedia) | ||
“Pinnularia are elongated elliptical unicellular organisms.” “Their walls are composed of two halves called thecae (or less formally, valves.)” (wikipedia) | “Pinnularia are elongated elliptical unicellular organisms.” “Their walls are composed of two halves called thecae (or less formally, valves.)” (wikipedia) | ||
====Medium==== | |||
<gallery> | |||
File:plant-fertilizer.JPG|fertilizer | |||
File:diatoms-medium.jpg | |||
</gallery> | |||
==bacteria== | ==bacteria== | ||
===Spirulina (Arthrospira platensis)=== | ===Spirulina (Arthrospira platensis)=== | ||
[[File:Arthrospira_cyanodb.cz.jpg|400px|thumb|Arthrospira. Source: cyanodb.cz]] | |||
====Classification==== | |||
*Domain: Bacteria | *Domain: Bacteria | ||
*Phylum: Cyanobacteria | *Phylum: Cyanobacteria | ||
*Genus: Spirulina | *Genus: Spirulina | ||
====Description==== | |||
“Cyanobacteria is a phylum of bacteria that obtain their energy through photosynthesis” | “Cyanobacteria is a phylum of bacteria that obtain their energy through photosynthesis” | ||
“Cyanobacteria used to be called blue-green algae but they are prokaryotes and the term "algae" in modern usage refers to eukaryotes.” (wikipedia) | “Cyanobacteria used to be called blue-green algae but they are prokaryotes and the term "algae" in modern usage refers to eukaryotes.” (wikipedia) | ||
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“The optimal growth temperature for A. platensis is 35 – 38 °C. This poses a major limiting factor outside the tropics, confining growth to the summer months.[22] A. platensis has been grown in fresh water, as well as in brackish water and sea water.” (wikipedia) | “The optimal growth temperature for A. platensis is 35 – 38 °C. This poses a major limiting factor outside the tropics, confining growth to the summer months.[22] A. platensis has been grown in fresh water, as well as in brackish water and sea water.” (wikipedia) | ||
====Medium==== | |||
"The water need to be pure for the reasons we just explained. This doesn’t mean that you have to use bottle water but you should filter the tap water – something like a Brita filter will do just fine." "If you use river water, use UV filters in addition to active carbon filter(the Brita type), as you might have other algae or organisms in the water that could compete or eat spirulina."(http://www.spirulinaacademy.com/how-to-grow-your-own-spirulina-at-home-the-culture-medium/) | |||
"Spirulina requires relatively high pH values between 9.5 and 9.8 ( Belkin and Boussiba, 1971)" | |||
*[http://www.spirulinaacademy.com/how-to-grow-your-own-spirulina-at-home-the-culture-medium/ Zarrouk Medium] | |||
*[http://justspirulina.org/spirulina-manual/ Simplified Medium + instructions] | |||
==Protists== | ==Protists== | ||
===Euglena=== | ===Euglena=== | ||
[[File:Euglena_wikimedia.org.jpg|400px|thumb|Euglena. Source: wikimedia.org]] | |||
====Classification==== | |||
*Domain: Eukaryota | *Domain: Eukaryota | ||
*Class: Euglenoidea | *Class: Euglenoidea | ||
*Genus: Euglena | *Genus: Euglena | ||
====Description==== | |||
Euglena is a (endo)symbiotic creature. It is also considered belonging to protista kingdom, having features of animal, plant or fungus. | Euglena is a (endo)symbiotic creature. It is also considered belonging to protista kingdom, having features of animal, plant or fungus. | ||
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“Most species of Euglena have photosynthesizing chloroplasts within the body of the cell, which enable them to feed by autotrophy, like plants. However, they can also take nourishment heterotrophically, like animals.” (wikipedia) | “Most species of Euglena have photosynthesizing chloroplasts within the body of the cell, which enable them to feed by autotrophy, like plants. However, they can also take nourishment heterotrophically, like animals.” (wikipedia) | ||
====Medium==== | |||
"Combine 1 L of spring water or Chalkley’s 1x solution or Pringsheim’s, 20 wheat or rice grains, and 5 mL (1 tsp) of dry | |||
powdered milk in a beaker. Boil the mixture for 5–10 minutes, and then dilute this mixture with 3 L of spring water. Let this solution cool and stand uncovered for 24 hours." | |||
"Shake or stir the media solution and fill shallow containers with wide bottoms approximately half full. (Stacking culture bowls work best.) Add 50–100 mL of Euglena stock culture to each bowl. The Euglena culture medium contains a great deal of organic matter. Bacteria will grow rapidly and the cultures are likely to have a pungent odor for several days. (Take this into consideration when selecting a culture storage location.)" | |||
Chalkley’s Stock Solution 10x (Dilute by a factor of 10 for 1x (e.g., dilute 100 mL to 1 L with distilled water)) | |||
0.06 g CaCl2 | |||
1.00 g NaCl | |||
0.04 g KCl | |||
1 L Distilled water | |||
Autotrophic/mixotrophic euglena medium: https://www.uni-weimar.de/kunst-und-gestaltung/wiki/File:Euglenamedien.pdf | |||
More info: [[:File:Culturing-Euglena.pdf]] |