GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The supermarket cloning project: Difference between revisions

GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The supermarket cloning project (view source)

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-Abstract:
+'''Abstract'''
+Specimen of mushrooms obtained from weimerian supermarkets are cloned and purified, than stored in a cold-storage strain-bank.
+Via reverse-engineering I extract the strand from the product sold. The strands will be available at an exhibition.
+'''Methodology'''
+Seven dishes per strand. PDA- medium. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish.
+The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, it is inserted in the hole that the hot scalpel made.
-Methodology:
+The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till clean and strong. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag.
-Reference: