140
edits
GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The supermarket cloning project: Difference between revisions
GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner/The supermarket cloning project (view source)
Revision as of 19:18, 28 January 2018
, 28 January 2018no edit summary
Jan Glöckner (talk | contribs) No edit summary |
Jan Glöckner (talk | contribs) No edit summary |
||
| Line 4: | Line 4: | ||
Via reverse-engineering I extract the strand from the product sold. The strands will be available at an exhibition. | Via reverse-engineering I extract the strand from the product sold. The strands will be available at an exhibition. | ||
''' | '''Method''' | ||
Seven dishes per strand. PDA- medium. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. | Seven dishes per strand. PDA- medium. The scalpel is sterilized in a hot flame till it glows red. It then is cooled in the receiving petri dish. | ||
The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, it is inserted in the hole that the hot scalpel made. | The mushroom is ripped apart in the middle. Tissue then is taken from just above the gills, it is inserted in the hole that the hot scalpel made. | ||
The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till clean and strong. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag. | The dishes are labeled and incubated at room temperature (23°-25° C). The mycelium that emerges is purified by running it through subsequent petri dishes till clean and strong. Then it is stored in test-tubes filled with PDA- medium at temperatures below 5°C. Two tubes per strand, enveloped in a zip-loc-bag. | ||