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Materials: Flat jar, wadding, agar-agar, malt extract powder, marker, distilled water, pressure cooker, precision scale (0,1 g), aluminum foil. | Materials: Flat jar, wadding, agar-agar, malt extract powder, marker, distilled water, pressure cooker, precision scale (0,1 g), aluminum foil. | ||
<u> | <u>Step 1: Calculate Ingredient Ratios</u> | ||
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<u> | <u>Step 2: Weigh Ingredients And Mix Into Flask</u> | ||
* Use a scale to measure the required ingredients accurately. | * Use a scale to measure the required ingredients accurately. | ||
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* Seal the jars and cover the lids with aluminum foil to prevent microbes from reaching the air filter later on. | * Seal the jars and cover the lids with aluminum foil to prevent microbes from reaching the air filter later on. | ||
<u> | <u>Step 3: Sterilize In Pressure Cooker</u> | ||
* Add a few centimeters of water into the pressure cooker, ensuring the vessels are submerged but not floating. Close the cooker and reach the designated temperature and pressure point (121°C, 15 PSI). (Avoid using airtight containers in the pressure cooker to prevent bursting; always refer to the pressure cooker manual for proper usage.) | * Add a few centimeters of water into the pressure cooker, ensuring the vessels are submerged but not floating. Close the cooker and reach the designated temperature and pressure point (121°C, 15 PSI). (Avoid using airtight containers in the pressure cooker to prevent bursting; always refer to the pressure cooker manual for proper usage.) | ||
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* Let the jars cool down naturally before storing them in the fridge. | * Let the jars cool down naturally before storing them in the fridge. | ||
<u> | <u>Step 4: Add Mycelium & Incubate</u> | ||
<gallery widths="100" heights="100"> | <gallery widths="100" heights="100"> | ||
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Objective: Prepare a sterile small bag to expand mycelium for fructification, fabrication, or as an inoculant for larger vessels. | Objective: Prepare a sterile small bag to expand mycelium for fructification, fabrication, or as an inoculant for larger vessels. | ||
Materials for 1 bag: See below, bucket that can hold 5 L (for mixing), jar with clean grain spawn, substrate, '''alcohol(?)''', autoclavable plastic bags, gloves, distilled water (approx. 2,5x the dry weight of the substrate). | Materials for 1 bag: See below, bucket that can hold 5 L (for mixing), jar with clean grain spawn, substrate, '''alcohol(?)''', autoclavable plastic bags, gloves, distilled water (approx. 2,5x the dry weight of the substrate).# | ||
<u>Step 1: Weigh the Substrate</u> | |||
<u>Step 2: Sterilise</u> | |||
<u>Step 3: Mix In Mycelium</u> | |||
<u>Step 4: Incubate</u> | |||
For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate] | For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-small-mycelium-bags-v.2.0-1.pdf Preparation of a Small Bag with Substrate] | ||