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'''To grow them in the laboratory''', we needed to reproduce their living environment and feed them. | '''To grow them in the laboratory''', we needed to reproduce their living environment and feed them. | ||
We cultivated | We cultivated the specie ''Hypsibius exemplaris'' in a 25ml plastic bottle. | ||
We cultivated separately a culture of the alga "Chlorococcum" in a tube containing spring water (Volvic) and 0,01% of fertilizer (Algabloom). We kept in them in the tent for the algae, with 12h light exposure cycles. | |||
Once a week, we fed the algae to the tardigrades as follows (each step needs a different sterile pipette tip): | Once a week, we fed the algae to the tardigrades as follows (each step needs a different sterile pipette tip): | ||
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- Refill the Chlorococcus culture with sterile cultivation medium (spring water with 0,01% of fertilizer algabloom) | - Refill the Chlorococcus culture with sterile cultivation medium (spring water with 0,01% of fertilizer algabloom) | ||
To observe them, with a sterile tip, take 200µl of water in the biomass at the bottom, put them on a slide and use the 10x magnification lens of the microscope to see them. [[File:In the lab.png|thumb|disolving the sample]] | |||