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GMU:Life in an aquatic ecosystem/Dahye Seo (view source)
Revision as of 09:48, 24 November 2024
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I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR. | I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR. | ||
Centrifuge setting: 8kg, 60 seconds | # Centrifuge setting: 8kg, 60 seconds | ||
# PCR: see instructional photo above A total of five DNA samples were subjected to DNA amplification by PCR | |||
PCR: see instructional photo above | |||
A total of five DNA samples were subjected to DNA amplification by PCR | |||
*Label information | *Label information | ||
*#B11 : DNA of Blueberries (from 19.Nov) | *#B11: DNA of Blueberries (from 19.Nov) | ||
*#B12 : DNA of Blueberries (from 19.Nov) | *#B12: DNA of Blueberries (from 19.Nov) | ||
*#B2: DNA of Blueberries (from 21.Nov) | *#B2: DNA of Blueberries (from 21.Nov) | ||
*#BA : DNA of Banana (from 21.Nov) | *#BA: DNA of Banana (from 21.Nov) | ||
*#K : Kaki pulp with soap, salt, hot water (from 20.Nov) | *#K: Kaki pulp with soap, salt, hot water (from 20.Nov) | ||
<small>*sample B11, B12 are dirtier than B2</small> | <small>*sample B11, B12 are dirtier than B2</small> | ||
* So far no success to extract Kaki DNA. I used this time kaki pulp instead of DNA. It seems like there are no DNA in pulp, but at least I wanted experiment with pulp until Gel-electrophoresis just for double checking. | |||
<gallery> | <gallery> | ||
File:Centrifugation1.jpg | File:Centrifugation1.jpg | ||