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===Isolation of Streptomyces=== | |||
:'''1st step''': Air dry the soil samples for ''3-4 h at 45°'' for the selection of Actinomyces spores, considering that many other bacteria die during this pre-treatment | |||
:'''2nd step''':Dilute ''1-3g of soil in 10 ml saline solution'' (NaCl 0,9%) and let the solution stand for ''15 min'' | |||
:'''3rd step''': Prepare 3 dilutions ''(1:10, 1:100, 1:1000)'' out of it | |||
:4th step: Prepare the Mannitol Soy Agar (MSA) petri dishes | |||
MSA Recipe: | |||
In a '''250 mL conical flask''' mix: | |||
*2g Agar | |||
*2g Mannitol | |||
*2g Soya flour (Holland and Barrett) | |||
*10 mM CaCl2 | |||
*100 mL Deionised water | |||
'''Inspiration''': | '''Inspiration''': | ||
http://annadumitriu.tumblr.com/ - Anna Dumitriu | http://annadumitriu.tumblr.com/ - Anna Dumitriu | ||
https://exploringtheinvisible.com/ - Dr. Simon Park | https://exploringtheinvisible.com/ - Dr. Simon Park |
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