GMU:BioArt WS16/Transgenic organisms: Difference between revisions

TSS medium reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the eis and then heat shock with 42 degrees warmed up water.

Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night