Bureaucrats, emailconfirmed, Administrators
5,468
edits
GMU:BioArt WS16/Transgenic organisms (view source)
Revision as of 12:06, 4 January 2017
, 4 January 2017→GFP E.coli Workshop (method 1)
| Line 73: | Line 73: | ||
Needed: | Needed: | ||
* ( | * (Reptile) incubator | ||
* Spectrometer to calculate cell growth mass | * Spectrometer to calculate cell growth mass | ||
TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water. | TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water. | ||